Overview of Pyridine Nucleotides Review Series

The stability of pyridine nucleotides.

Diphosphopyridine nucleotide and triphosphopyridine nucleotide when used in appropriate enzyme systems become exceedingly useful reagents for the measurement of almost any biochemical substance. A companion paper describes procedures for measuring each of the pyridine nucleotides, whether in the oxidized or reduced form, at concentrations as low as 1O-g M and in amounts as small as lo-l5 moles ...

Pyridine nucleotides in erythrocyte metabolism.

Streptozotocin: depression of mouse liver pyridine nucleotides.

phenomenon readily prevented by nicotinamide pretreatment (21). This communication deals with the changes in liver NAD and reduced nicotinamide adenine dinucleotide (NADH) con centrations over a 48-hour period following the single intra venous administration of Streptozotocin. These effects are cor related with the measurement of drug concentration in serum and the acid-soluble fraction of live...

A structure of pyridine nucleotides in solution.

Re-examination of the structure of pyridine coenzymes in solution by use of the 220-MHz high-frequency nuclear magnetic resonance spectrometer indicates that there is primarily one folded structure that is in rapid equilibrium with an open form. Reduced DPN(+) and reduced analogs of DPN(+) exist predominantly with the B side of the dihydropyridine ring folded against the adenine moiety. (The ox...

Oxidation of pyridine nucleotides coupled to phosphorylation.

Previous studies have indicated that the pentose cycle enzymes, including glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase, were localized in the soluble fraction of the homogenate isolated from kidney, liver, or heart tissue (1). Since reduced pyridine nucleotides are formed during the oxidation of G-6-P’ and 6-PGA, it was of interest to establish whether or not the reoxi...